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  2. Structured illumination light sheet microscopy - Wikipedia

    en.wikipedia.org/wiki/Structured_Illumination...

    Moiré Effect. Structured illumination microscopy (SIM) is a method of super-resolution microscopy which is performed by acquiring multiple images of the same sample under different patterns of illumination, then computationally combining these images to achieve a single reconstruction with up to 2x improvement over the diffraction limited lateral resolution.

  3. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  4. Köhler illumination - Wikipedia

    en.wikipedia.org/wiki/Köhler_illumination

    Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.

  5. Lattice light-sheet microscopy - Wikipedia

    en.wikipedia.org/wiki/Lattice_light-sheet_microscopy

    Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...

  6. Super-resolution photoacoustic imaging - Wikipedia

    en.wikipedia.org/wiki/Super-resolution_photo...

    Structured illumination is an imaging technique that when applied to microscopy, can double the spatial resolution of that of conventional fluorescence microscopy using the moiré interference pattern, the coarse pattern that is produced when two finer patterns are overlapped and provides easier viewing than either original pattern. [8]

  7. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...

  8. Optical heterodyne detection - Wikipedia

    en.wikipedia.org/wiki/Optical_heterodyne_detection

    A form of super-resolution microscopy, this work continues to spawn a family and generation of microscopes of particular use in the life sciences, known as "structured illumination microscopy", Polaroid Corp. patented Guerra's invention in 1997. [5]

  9. Vertico spatially modulated illumination - Wikipedia

    en.wikipedia.org/wiki/Vertico_spatially...

    SMI + TIRF of human eye tissue affected by macular degeneration SMI microscopy is a light optical process of the so-called point spread function-engineering.These are processes which modify the point spread function (PSF) of a microscope in a suitable manner to either increase the optical resolution, to maximize the precision of distance measurements of fluorescent objects that are small ...

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