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Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH + 4) and sulfate (SO 2− 4) in aqueous solutions. [1] Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive.
Ammonium sulfate precipitation is a common method for protein purification by precipitation. As the ionic strength of a solution increases, the solubility of proteins in that solution decreases. Being extremely soluble in water, ammonium sulfate can "salt out" (precipitate) proteins from aqueous solutions.
z i is the ion charge of the salt and c i is the salt concentration. The ideal salt for protein precipitation is most effective for a particular amino acid composition, inexpensive, non-buffering, and non-polluting. The most commonly used salt is ammonium sulfate. There is a low variation in salting out over temperatures 0 °C to 30 °C.
The following chart shows the solubility of various ionic compounds in water at 1 atm pressure and room temperature (approx. 25 °C, 298.15 K). "Soluble" means the ionic compound doesn't precipitate, while "slightly soluble" and "insoluble" mean that a solid will precipitate; "slightly soluble" compounds like calcium sulfate may require heat to precipitate.
Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) [1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.
Ammonium iron(II) sulfate, or Mohr's salt, is the inorganic compound with the formula (NH 4) 2 SO 4 ·Fe(SO 4)·6H 2 O. Containing two different cations, Fe 2+ and NH + 4, it is classified as a double salt of ferrous sulfate and ammonium sulfate. It is a common laboratory reagent because it is readily crystallized, and crystals resist oxidation ...
Ammonium chlorate is an inorganic compound with the formula NH 4 ClO 3.. It is obtained by neutralizing chloric acid with either ammonia or ammonium carbonate, or by precipitating barium, strontium or calcium chlorates with ammonium carbonate or ammonium sulfate, producing the respective carbonate or sulfate precipitate and an ammonium chlorate solution.
Silver phosphate is formed as a yellow solid precipitate by the reaction between a soluble silver salt, such as silver nitrate, with a soluble orthophosphate. [3] Its solubility product is 8.89×10 −17 mol 4 ·dm −2. [4] [5] The precipitation reaction is analytically significant [6] and can be used in qualitative or quantitative analysis. [7]
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