Ads
related to: working principle of flow cytometryqbd.creative-diagnostics.com has been visited by 10K+ users in the past month
macrophage.creative-biolabs.com has been visited by 10K+ users in the past month
Search results
Results from the WOW.Com Content Network
The first impedance-based flow cytometry device, using the Coulter principle, was disclosed in U.S. Patent 2,656,508, issued in 1953, to Wallace H. Coulter.Mack Fulwyler was the inventor of the forerunner to today's flow cytometers – particularly the cell sorter. [6]
Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells. The rapid growth in the multidimensionality and throughput ...
Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI).
A Coulter counter played an important role in the development of the first cell sorter, and was involved in the early development of flow cytometry. Some flow cytometers continue to utilize the Coulter principle to provide information about cell size and count.
Flow cytometry cell-sorting yields very high specificity according to one or several surface markers, but one limitation is constituted by the number of cells that can be processed during a work day. For this reason pre-enrichment of the population of interest by immunomagnetic cell sorting is often considered, especially when the target cells ...
Flow cytometry is by far the most sophisticated and expensive method for cell counting. In a flow cytometer the cells flow in a narrow stream in front of a laser beam. The beam hits them one by one, and a light detector picks up the light that is reflected from the cells.
Ads
related to: working principle of flow cytometryqbd.creative-diagnostics.com has been visited by 10K+ users in the past month
macrophage.creative-biolabs.com has been visited by 10K+ users in the past month