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The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture-based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the susceptibility ...
Mueller Hinton agar is commonly used in the disk diffusion method, which is a simple and widely used method for testing the susceptibility of bacterial isolates to antibiotics. In this method, small disks impregnated with different antibiotics are placed on the surface of the agar, and the zone of inhibition around each disk is measured to ...
The Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) provide standards for the type and depth of agar, temperature of incubation, and method of analysing results. [11] Disc diffusion is considered the cheapest and most simple of the methods used to test for susceptibility ...
The double-disc synergy test (DDST) utilizes two of these disks on the cultivated agar solution, either infused with a different antimicrobial solution. [ 2 ] This test was recommended the standard by the Clinical and Laboratory Standards Institute in 2004 for its use against MRSA . [ 3 ]
For some microbial and antimicrobial combinations, the results can be used to determine a minimum inhibitory concentration (MIC). Etest is a proprietary system manufactured by bioMérieux. It is a laboratory test used in healthcare settings to help guide physicians by indicating what concentration of antimicrobial could successfully be used to ...
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
"Chapter 12: Precipitation: Single Radial Immunodiffusion: Laboratory Technique 12-1: Radial Immunodiffusion Test". Essentials of Immunology & Serology. Albany, New York: Delmar Division of Thomson Learning. pp. 172– 174. ISBN 978-0914826255. LCCN 2002280630. OCLC 1149023866 – via Internet Archive.
Colonial morphology serves as the first step in the identification of microbial species from clinical samples. [10] Based on the visual appearance of the colonies, microbiologists can narrow down the list of possible organisms, allowing them to select appropriate tests to provide a definitive diagnosis.