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It belongs to the family of monomethine cyanine dyes and is a tetracationic homodimer of Oxazole Yellow (abbreviated YO, hence the name YOYO), typically available as tetraiodide salt. In aqueous buffer, free YOYO-1 dye (absorption: λ max 458 nm, emission: λ max 564 nm) has very low fluorescence quantum yield .
laser dye-doped bio-based gain media [4] Organic-inorganic nanoparticle gain media are nanocomposites developed for solid-state dye lasers [3] and can also be used in biosensors, [5] bioanalytics, [5] and nonlinear organic photonics applications. [6] An additional class of organic materials used in the generation of laser light include organic ...
(top) a 1,2,3,3-tetramethyl-3H-indolium salt and (bottom) aniline derivative of squaraine dyes. Squaraine dyes are a class of organic dyes showing intense fluorescence, typically in the red and near infrared region (absorption maxima are found between 630 and 670 nm and their emission maxima are between 650–700 nm).
A dye laser is a laser that uses an organic dye as the lasing medium, usually as a liquid solution. Compared to gases and most solid state lasing media, a dye can usually be used for a much wider range of wavelengths , often spanning 50 to 100 nanometers or more.
Photobleaching is an important parameter to account for in real-time single-molecule fluorescence imaging in biophysics. At light intensities used in single-molecule fluorescence imaging (0.1-1 kW/cm 2 in typical experimental setups), even most robust fluorophores continue to emit for up to 10 seconds before photobleaching in a single step. For ...
A growing number of dyes are used for PALM, STORM and related techniques, both organic fluorophores and fluorescent proteins. Some are compatible with live cell imaging, others allow faster acquisition or denser labeling. The choice of a particular fluorophore ultimately depends on the application and on its underlying photophysical properties. [5]
This improved gain medium was central to the demonstration of the first tunable narrow-linewidth solid-state dye laser oscillators, by Duarte, [8] which were later optimized to deliver pulse emission in the kW regime in nearly diffraction limited beams with single-longitudinal-mode laser linewidths of ≈ 350 MHz (or ≈ 0.0004 nm, at a laser wavelength of 590 nm). [9]
Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. When bound to DNA, acridine orange is very similar ...
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