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Laurdan is an organic compound which is used as a fluorescent dye when applied to fluorescence microscopy. [ 1 ] [ 2 ] It is used to investigate membrane qualities of the phospholipid bilayers of cell membranes .
Fluorescein is an organic compound and dye based on the xanthene tricyclic structural motif, formally belonging to triarylmethine dyes family. It is available as a dark orange/red powder slightly soluble in water and alcohol. It is used as a fluorescent tracer in many applications. [1]
Photobleaching is an important parameter to account for in real-time single-molecule fluorescence imaging in biophysics. At light intensities used in single-molecule fluorescence imaging (0.1-1 kW/cm 2 in typical experimental setups), even most robust fluorophores continue to emit for up to 10 seconds before photobleaching in a single step. For ...
Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium. Rhodamine dyes are also used extensively in biotechnology applications such as fluorescence microscopy , flow cytometry , fluorescence correlation ...
Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. When bound to DNA, acridine orange is very similar ...
Rhodamine dyes fluoresce and can thus be detected using Fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. [2] Rhodamine 123 is used in biochemistry to inhibit mitochondrion function.
The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific. [4] DyLight dyes are typically used in biotechnology and research applications as biomolecule , cell and tissue labels for fluorescence microscopy , cell biology or molecular biology .
Within the STED microscopy (STimulated Emission Depletion microscopy) [1] [2] a fluorescent dye molecule is driven between its electronic ground state and its excited state while sending out fluorescence photons. This is the standard operation mode in fluorescence microscopy and depicts state A.
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