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Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
The original interface between capillary zone electrophoresis and mass spectrometry was developed in 1987 [9] by Richard D. Smith and coworkers at Pacific Northwest National Laboratory, and who also later were involved in development of interfaces with other CE variants, including capillary isotachophoresis and capillary isoelectric focusing.
The apparatus consists of a vial with a conical bottom, a grounded platinum electrode, a capillary to inject the aqueous solution, and an adjustable gold anode with a circular bottom that contacts the entire organic phase. EE is also often performed in a capillary electrophoresis capillary. This is referred to as capillary electroextraction, or ...
The PCR products are quantified, typically by (capillary) electrophoresis. Each probe pair consists of two oligonucleotides, with sequence that recognizes adjacent sites of the target DNA , a PCR priming site, and optionally a "stuffer" to give the PCR product a unique length when compared to other probe pairs in the MLPA assay.
[1] [2] Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube , which is filled with the analyte.
It is a modification of capillary electrophoresis (CE), extending its functionality to neutral analytes, [1] where the samples are separated by differential partitioning between micelles (pseudo-stationary phase) and a surrounding aqueous buffer solution (mobile phase). [2]
Affinity capillary electrophoresis takes the advantages of capillary electrophoresis and applies them to the study of protein interactions. [16] ACE is advantageous because it has a high separation efficiency, has a shorter analysis time, can be run at physiological pH, and involves low consumption of ligand/molecules.
Microfluidic Sanger sequencing is a lab-on-a-chip application for DNA sequencing, in which the Sanger sequencing steps (thermal cycling, sample purification, and capillary electrophoresis) are integrated on a wafer-scale chip using nanoliter-scale sample volumes. This technology generates long and accurate sequence reads, while obviating many ...