Ads
related to: next generation sequencing steps
Search results
Results from the WOW.Com Content Network
This design is very different from that of Sanger sequencing—also known as capillary sequencing or first-generation sequencing—which is based on electrophoretic separation of chain-termination products produced in individual sequencing reactions. [6] This methodology allows sequencing to be completed on a larger scale. [7]
2 Base Encoding, also called SOLiD (sequencing by oligonucleotide ligation and detection), is a next-generation sequencing technology developed by Applied Biosystems and has been commercially available since 2008. These technologies generate hundreds of thousands of small sequence reads at one time.
The first of the high-throughput sequencing technologies, massively parallel signature sequencing (or MPSS, also called next generation sequencing), was developed in the 1990s at Lynx Therapeutics, a company founded in 1992 by Sydney Brenner and Sam Eletr. MPSS was a bead-based method that used a complex approach of adapter ligation followed by ...
RNA-Seq was first developed in mid 2000s with the advent of next-generation sequencing technology. [144] The first manuscripts that used RNA-Seq even without using the term includes those of prostate cancer cell lines [ 145 ] (dated 2006), Medicago truncatula [ 146 ] (2006), maize [ 147 ] (2007), and Arabidopsis thaliana [ 148 ] (2007), while ...
The shotgun strategy is still applied today, however using other sequencing technologies, such as short-read sequencing and long-read sequencing. Short-read or "next-gen" sequencing produces shorter reads (anywhere from 25–500bp) but many hundreds of thousands or millions of reads in a relatively short time (on the order of a day). [18]
SOLiD (Sequencing by Oligonucleotide Ligation and Detection) is a next-generation DNA sequencing technology developed by Life Technologies and has been commercially available since 2006. This next generation technology generates 10 8 - 10 9 small sequence reads at one time.
Ads
related to: next generation sequencing steps