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Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing.
Single-cell RNA sequencing workflow. Current scRNA-seq protocols involve isolating single cells and their RNA, and then following the same steps as bulk RNA-seq: reverse transcription (RT), amplification, library generation and sequencing. Early methods separated individual cells into separate wells; more recent methods encapsulate individual ...
Prior to this, only transcriptomes of organisms that were of broad interest and utility to scientific research were sequenced; however, these developed in 2010s high-throughput sequencing (also called next-generation sequencing) technologies are both cost- and labor- effective, and the range of organisms studied via these methods is expanding. [2]
RNA-Seq was first developed in mid 2000s with the advent of next-generation sequencing technology. [144] The first manuscripts that used RNA-Seq even without using the term includes those of prostate cancer cell lines [ 145 ] (dated 2006), Medicago truncatula [ 146 ] (2006), maize [ 147 ] (2007), and Arabidopsis thaliana [ 148 ] (2007), while ...
Duplex sequencing library preparation workflow: ... A method for counting PCR template molecules with application to next-generation sequencing is an example. [1]
The workflow of a typical hybrid genome assembly experiment using second- and third-generation sequencing technologies. Figure adapted from Wang et al., 2012 [14]. One hybrid approach to genome assembly involves supplementing short, accurate second-generation sequencing data (i.e. from IonTorrent, Illumina or Roche 454) with long less accurate third-generation sequencing data (i.e. from PacBio ...
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