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Condensers typically consist of a variable-aperture diaphragm and one or more lenses. Light from the illumination source of the microscope passes through the diaphragm and is focused by the lens(es) onto the specimen. After passing through the specimen the light diverges into an inverted cone to fill the front lens of the objective.
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
A bright-field microscope has many important parts including; the condenser, the objective lens, the ocular lens, the diaphragm, and the aperture. Some other pieces of the microscope that are commonly known are the arm, the head, the illuminator, the base, the stage, the adjusters, and the brightness adjuster.
The centre of the diaphragm's aperture coincides with the optical axis of the lens system. Most modern cameras use a type of adjustable diaphragm known as an iris diaphragm, and often referred to simply as an iris. See the articles on aperture and f-number for the photographic effect and system of quantification of varying the opening in the ...
Huygens eyepiece diagram. Huygens eyepieces consist of two plano-convex lenses with the plane sides towards the eye separated by an air gap. The lenses are called the eye lens and the field lens. The focal plane is located between the two lenses. It was invented by Christiaan Huygens in the late 1660s and was the first compound (multi-lens ...
In a ray diagram of the illumination light path, this can be seen as the image-forming rays passing parallel through the sample. Köhler illumination requires several optical components to function: Collector lens and/or field lens; Field diaphragm; Condenser diaphragm; Condenser lens; Schematics of Köhler illumination.
The success of the phase-contrast microscope has led to a number of subsequent phase-imaging methods. In 1952, Georges Nomarski patented what is today known as differential interference contrast (DIC) microscopy. [8] It enhances contrast by creating artificial shadows, as if the object is illuminated from the side.
Léon Foucault developed a catadioptric microscope in 1859 to counteract aberrations of using a lens to image objects at high power. [2] In 1876 a French engineer, A. Mangin, invented what has come to be called the Mangin mirror, a concave glass reflector with the silver surface on the rear side of the glass. The two surfaces of the reflector ...