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There are other hybrid ionization methods which combine resonant or non-resonant laser desorption with post electrospray ionization. One example is electrospray laser desorption ionization (ELDI), which uses an ultraviolet laser to form ions by irradiating the sample directly, then interacting with the electrospray plume without using any ...
The AP-MALDI ion source is easily coupled to an ion trap mass spectrometer [34] or any other MS system equipped with electrospray ionization (ESI) or nanoESI source. MALDI with ionization at reduced pressure is known to produce mainly singly-charged ions (see "Ionization mechanism" below).
Extractive electrospray ionization is a spray-type, ambient ionization method that uses two merged sprays, one of which is generated by electrospray. [ 49 ] Laser-based electrospray-based ambient ionization is a two-step process in which a pulsed laser is used to desorb or ablate material from a sample and the plume of material interacts with ...
In 2001, more than 1700 papers on proteomics were published, many using electrospray ionization. [9] Electrospray ionization provides a way to get accurate information about the mass of a large molecule very quickly, even when it is in a mixture of other molecules. [10] The liquid sample is introduced into an electrospray source (at atmospheric ...
Scheme of inlet ionization. In mass spectrometry, matrix-assisted ionization (also inlet ionization) is a low fragmentation (soft) ionization technique which involves the transfer of particles of the analyte and matrix sample from atmospheric pressure (AP) to the heated inlet tube connecting the AP region to the vacuum of the mass analyzer.
Diagram of a MALDI ion source. Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique. The sample is mixed with a matrix material. Upon receiving a laser pulse, the matrix absorbs the laser energy and it is thought that primarily the matrix is desorbed and ionized (by addition of a proton) by this event.
Desorption electrospray ionization (DESI) is an ambient ionization technique that can be coupled to mass spectrometry (MS) for chemical analysis of samples at atmospheric conditions. Coupled ionization sources-MS systems are popular in chemical analysis because the individual capabilities of various sources combined with different MS systems ...
[1] [2] In MALDI, the sample is mixed with a matrix material and applied to a metal plate before irradiation by a laser, [3] whereas in SELDI, proteins of interest in a sample become bound to a surface before MS analysis. The sample surface is a key component in the purification, desorption, and ionization of the sample.