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The western blot is routinely used for verification of protein production after cloning. It is also used in medical diagnostics, e.g., in the HIV test or BSE-Test. [9] The confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample.
Oligoclonal bands (OCBs) are bands of immunoglobulins that are seen when a patient's blood serum, or cerebrospinal fluid (CSF) is analyzed. They are used in the diagnosis of various neurological and blood diseases. Oligoclonal bands are present in the CSF of more than 95% of patients with clinically definite multiple sclerosis. [1]
Diagnosing Lyme borreliosis relies on clinical criteria, with a history of a tick bite and associated symptoms being crucial. Laboratory diagnosis follows a 'two-tiered diagnostic protocol,' involving detecting specific antibodies using methods such as immunoenzymatic assays and Western blot tests, preferably with recombinant antigens.
With Lyme disease, this condition is often bilateral, meaning it happens on both sides of the face. If the disease spreads to the joints, it can cause arthritis in the knee, the wrist, and the ankles. Lyme disease can also spread to the meninges, which is the lining of the brain, causing meningitis and sometimes terrible neck stiffness and ...
Lyme disease, also known as Lyme borreliosis, is a tick-borne disease caused by species of Borrelia bacteria, transmitted by blood-feeding ticks in the genus Ixodes. [ 4 ] [ 9 ] [ 10 ] The most common sign of infection is an expanding red rash , known as erythema migrans (EM), which appears at the site of the tick bite about a week afterwards ...
Upon completion, a detection mechanism such as western blotting can be used, which will reveal the presence of bands. Each band represents a specific protein. The distance of travel is solely based on molecular weight; therefore, the molecular weight of each protein can be determined by comparing the distance of an unknown protein to the ...
Borrelia burgdorferi is a bacterial species of the spirochete class in the genus Borrelia, and is one of the causative agents of Lyme disease in humans. [1] [2] Along with a few similar genospecies, some of which also cause Lyme disease, it makes up the species complex of Borrelia burgdorferi sensu lato.
DNA bands are separated by size. DNA bands are stained. Gel electrophoresis is an electrophoresis method for separation and analysis of biomacromolecules ( DNA , RNA , proteins , etc.) and their fragments, based on their size and charge through a gel .