Search results
Results from the WOW.Com Content Network
MALDI mass spectrometry imaging (MALDI-MSI) is the use of matrix-assisted laser desorption ionization as a mass spectrometry imaging [2] technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is recorded. [3]
The term matrix-assisted laser desorption ionization (MALDI) was coined in 1985 by Franz Hillenkamp, Michael Karas and their colleagues. [3] These researchers found that the amino acid alanine could be ionized more easily if it was mixed with the amino acid tryptophan and irradiated with a pulsed 266 nm laser.
More than 50 years ago, MSI was introduced using secondary ion mass spectrometry (SIMS) to study semiconductor surfaces by Castaing and Slodzian. [5] However, it was the pioneering work of Richard Caprioli and colleagues in the late 1990s, demonstrating how matrix-assisted laser desorption/ionization (MALDI) could be applied to visualize large biomolecules (as proteins and lipids) in cells and ...
The schematic of IR-MALDESI imaging source. The IR-MALDESI source can be used for mass spectrometry imaging (MSI), a technique using MS data analyzed from a sample area to detect hundreds to thousands of biomolecules and visualize their spatial distributions.
In mass spectrometry, a matrix is a compound that promotes the formation of ions. Matrix compounds are used in matrix-assisted laser desorption/ionization (MALDI), matrix-assisted ionization (MAI), and fast atom bombardment (FAB).
MS 2 – Mass spectrometry/mass spectrometry, i.e. tandem mass spectrometry; MS/MS – Mass spectrometry/mass spectrometry, i.e. tandem mass spectrometry; MALDESI – Matrix-assisted laser desorption electrospray ionization; MALDI – Matrix-assisted laser desorption/ionization; MAII – Matrix-assisted inlet ionization; MAIV – Matrix ...
Mass spectrometric immunoassay (MSIA) is a rapid method is used to detect and/ or quantify antigens and or antibody analytes. [1] This method uses an analyte affinity (either through antigens or antibodies) isolation to extract targeted molecules and internal standards from biological fluid in preparation for matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI ...
More recently, another major contribution to the field of mass spectrometry was his application of MALDI mass spectrometry for imaging of biological samples. [3] First published in 1997, the technique has been widely adopted and has been used to visualize proteins, peptides, drugs and other small molecules in tissue and other biological materials.