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Salmonella species: red colonies, some with black centers. The agar itself will turn red due to the presence of Salmonella type colonies. Shigella species: red colonies. Coliforms: yellow to orange colonies. Pseudomonas aeruginosa: pink, flat, rough colonies. This type of colony can be easily mistaken for Salmonella due to the color similarities.
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Salmonella species can be found in the digestive tracts of humans and animals, especially reptiles. Salmonella on the skin of reptiles or amphibians can be passed to people who handle the animals. [39] Food and water can also be contaminated with the bacteria if they come in contact with the feces of infected people or animals. [40]
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
Salmonella bongori was previously considered a subspecies of S. enterica, but it is now the other species in the genus Salmonella. Most of the human pathogenic Salmonella serovars belong to the enterica subspecies. These serogroups include S. Typhi, S. Enteritidis, S. Paratyphi, S. Typhimurium, and S. Choleraesuis.
The streak plate method [2] helps identify the unknown microbe by producing individual colonies on an agar plate which allows for CFU method to be used: Beginning the streak pattern. Label the base of the plate. Then, visualize the plate in four quadrants: top left (I), top right (II), bottom right (III), bottom left (IV).
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars). Bismuth sulfite agar is a type of agar media used to isolate Salmonella species.
This bacteria would grow on culture medium so there might have been a particular reason why they were grown on a human-cell culture for the SEM in question. If this image appeared in a journal, it presume the paper would also include how the specimen for SEM was prepared, including any materials used, what the human cells are, what parts of the ...