Search results
Results from the WOW.Com Content Network
Conditional gene knockout is a technique used to eliminate a specific gene in a certain tissue, such as the liver. [ 1 ][ 2 ] This technique is useful to study the role of individual genes in living organisms. It differs from traditional gene knockout because it targets specific genes at specific times rather than being deleted from beginning ...
Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic ...
A conditional gene knockout allows gene deletion in a tissue in a tissue specific manner. This is required in place of a gene knockout if the null mutation would lead to embryonic death, [12] or a specific tissue or cell type is of specific interest. This is done by introducing short sequences called loxP sites around the gene.
The term "floxing" is a portmanteau constructed from the phrase "flanking/flanked by LoxP". The floxing method is essential in the development of scientific model systems as it allows researchers to have spatial and temporal alteration of gene expression. [ 2 ] The Cre-Lox system is widely used to manipulate gene expression in model organisms ...
Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I -like mechanism to carry out site specific recombination events. The enzyme (38 kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two ...
Gene knock-in originated as a slight modification of the original knockout technique developed by Martin Evans, Oliver Smithies, and Mario Capecchi.Traditionally, knock-in techniques have relied on homologous recombination to drive targeted gene replacement, although other methods using a transposon-mediated system to insert the target gene have been developed. [3]
Gene targeting. A chimeric mouse gene targeted for the agouti coat color gene, with its offspring. Gene targeting is a biotechnological tool used to change the DNA sequence of an organism (hence it is a form of Genome Editing). It is based on the natural DNA-repair mechanism of Homology Directed Repair (HDR), including Homologous Recombination.
A blue rose is a flower of the genus Rosa (family Rosaceae) that presents blue-to-violet pigmentation instead of the more common red, white, or yellow, through use of artificial means such as dyes. Blue roses are often used to symbolize mystery or the unattainable, [1] since they do not exist in nature because of genetic limitations.