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Boric acid in equilibrium with its conjugate base the borate ion is widely used (in the concentration range 50–100 ppm boron equivalents) as a primary or adjunct pH buffer system in swimming pools. Boric acid is a weak acid, with pK a (the pH at which buffering is strongest because the free acid and borate ion are in equal concentrations) of ...
A mixture containing citric acid, monopotassium phosphate, boric acid, and diethyl barbituric acid can be made to cover the pH range 2.6 to 12. [ 8 ] Other universal buffers are the Carmody buffer [ 9 ] and the Britton–Robinson buffer , developed in 1931.
Borate buffered saline (abbreviated BBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Borate buffers have an alkaline buffering capacity in the 8–10 range. Boric acid has a pK a of 9.14 at 25 °C.
Most of the carbonic acid then dissociates to bicarbonate and hydrogen ions. The bicarbonate buffer system is an acid-base homeostatic mechanism involving the balance of carbonic acid (H 2 CO 3), bicarbonate ion (HCO − 3), and carbon dioxide (CO 2) in order to maintain pH in the blood and duodenum, among other tissues, to support proper ...
This mixture consists of 0.0286 M citric acid, 0.0286 M monopotassium phosphate, 0.0286 M boric acid, 0.0286 M veronal and 0.0286 M hydrochloric acid titrated with 0.2 M sodium hydroxide. The buffer was invented in 1931 by the English chemist Hubert Thomas Stanley "Kevin" Britton (1892–1960) and the New Zealand chemist Robert Anthony Robinson ...
Borate ions (commonly supplied as boric acid) are used in biochemical and chemical laboratories to make buffers, e.g. for polyacrylamide gel electrophoresis of DNA and RNA, such as TBE buffer (borate buffered tris-hydroxymethylaminomethonium) [29] [30] or the newer SB buffer or BBS buffer (borate buffered
TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA.. In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis.
LB buffer, also known as lithium borate buffer, is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Lithium borate ( lithium hydroxide monohydrate and boric acid ).