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The mobile phase moves through the chromatography column (the stationary phase) where the sample interacts with the stationary phase and is separated. Preparative chromatography – the use of chromatography to purify sufficient quantities of a substance for further use, rather than analysis.
Typical stationary phases for normal-phase chromatography are silica or organic moieties with cyano and amino functional groups. For reversed phase, alkyl hydrocarbons are the preferred stationary phase; octadecyl (C18) is the most common stationary phase, but octyl (C8) and butyl (C4) are also used in some applications. The designations for ...
See also Aqueous normal phase chromatography. It is commonly believed that in HILIC, the mobile phase forms a water-rich layer on the surface of the polar stationary phase vs. the water-deficient mobile phase, creating a liquid/liquid extraction system. The analyte is distributed between these two layers.
The sample is deposited on the plate, which is eluted with a solvent or solvent mixture known as the mobile phase (or eluent). [3] This solvent then moves up the plate via capillary action. [4] As with all chromatography, some compounds are more attracted to the mobile phase, while others are more attracted to the stationary phase. [5]
Reverse phase high-performance liquid chromatography (RP-HPLC) involves a non-polar stationary phase, often a hydrocarbon chain, and a polar mobile or liquid phase. The mobile phase generally consists of an aqueous portion with an organic addition, such as methanol or acetonitrile .
The basic methods of separation in HPLC rely on a mobile phase (water, organic solvents, etc.) being passed through a stationary phase (particulate silica packings, monoliths, etc.) in a closed environment (column); the differences in reactivity among the solvent of interest and the mobile and stationary phases distinguish compounds from one ...
The mobile phase is generally a mixture of non-polar organic solvent, while the stationary phase is polar inorganic solvent water. Here, paper is used to support the stationary phase, water. Polar water molecules are held inside the void space of the cellulose network of the paper. The difference between TLC and paper chromatography is that the ...
Capillary electrochromatography (CEC) combines the principles used in HPLC and CE. The mobile phase is driven across the chromatographic bed using electroosmosis instead of pressure (as in HPLC). Electroosmosis is the motion of liquid induced by an applied potential across a porous material, capillary tube, membrane or any other fluid conduit.
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