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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released.
Crash Course (web series) Crash Course. (web series) Crash Course (sometimes stylized as CrashCourse) is an educational YouTube channel started by John Green and Hank Green (collectively the Green brothers ), who became known on YouTube through their Vlogbrothers channel. [2] [3] [4]
Biological engineering or bioengineering is the application of principles of biology and the tools of engineering to create usable, tangible, economically viable products. [1] Biological engineering employs knowledge and expertise from a number of pure and applied sciences, [2] such as mass and heat transfer, kinetics, biocatalysts ...
Organism. An organism is defined in a medical dictionary as any living thing that functions as an individual. [1] Such a definition raises more problems than it solves, not least because the concept of an individual is also difficult. Many criteria, few of them widely accepted, have been proposed to define what is an organism.
Transposon insertion sequencing ( Tn-seq) combines transposon insertional mutagenesis with massively parallel sequencing (MPS) of the transposon insertion sites to identify genes contributing to a function of interest in bacteria. The method was originally established by concurrent work in four laboratories under the acronyms HITS, [1] INSeq ...
Warburg effect (oncology) In oncology, the Warburg effect ( / ˈvɑːrbʊərɡ /) is the observation that most cancer cells release energy predominantly not through the 'usual' citric acid cycle and oxidative phosphorylation in the mitochondria as observed in normal cells, but through a less efficient process of 'anaerobic glycolysis ...
Uses. This method is useful for determining the activity of certain cells over a prolonged period of time. The method has been used to study protein kinase C, ubiquitin, and many other proteins. The method was also used to prove the existence and function of Okazaki fragments. George Palade used pulse-chase of radioactive amino acids to ...
Biological thermodynamics (Thermodynamics of biological systems) is a science that explains the nature and general laws of thermodynamic processes occurring in living organisms as nonequilibrium thermodynamic systems that convert the energy of the Sun and food into other types of energy. The nonequilibrium thermodynamic state of living ...