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In the context of microbiology, enucleation refers to removing the nucleus of a cell and replacing it with a different nucleus. This is used mainly in cloning but can also be used for creating hybrids of plants or animals. It was discovered that cells can be chemically enucleated with Cytochalasin B in 1967. [1]
Enucleation (surgery), the removal of a mass without cutting into or dissecting it Enucleation of the eye, removal of the eye that leaves the eye muscles and remaining orbital contents intact Self-enucleation, self-inflicted removal of the eye; Enucleation (microbiology), removing the nucleus of a cell and replacing it with a different nucleus
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This method, sometimes called enucleation, leaves behind the transparent exoskeleton so that clotting of haemolymph, and closure of the wound, may occur more rapidly. Cauterizing through the eyestalk with either an electrocautery device or an instrument such as a red-hot wire or forceps. If performed correctly, this method closes the wound and ...
Enucleation is the removal of the eye that leaves the eye muscles and remaining orbital contents intact. This type of ocular surgery is indicated for a number of ocular tumors , in eyes that have sustained severe trauma, and in eyes that are otherwise blind and painful.
They require less manual effort, give good sample recovery and are easy to clean between samples. Advantages of this technique are higher yields of proteins and nucleic acids from small, hard tissue samples - especially when used as a preliminary step to mechanical or chemical/solvent cell disruption methods mentioned above.
These chemotherapeutics help to prevent failure of the filter bleb from scarring by inhibiting fibroblast proliferation. Alternatively, non-chemotherapeutic adjuvants can be implemented to prevent super scarring by wound modulation, such as the ologen collagen matrix implant.
In general, deliberately induced immunosuppression is performed to prevent the body from rejecting an organ transplant. [3] Additionally, it is used for treating graft-versus-host disease after a bone marrow transplant , or for the treatment of auto-immune diseases such as systemic lupus erythematosus , rheumatoid arthritis , Sjögren's ...