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Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]
Types of mutations that can be introduced by random, site-directed, combinatorial, or insertional mutagenesis. In molecular biology, mutagenesis is an important laboratory technique whereby DNA mutations are deliberately engineered to produce libraries of mutant genes, proteins, strains of bacteria, or other genetically modified organisms.
Gene knockout by mutation is commonly carried out in bacteria. An early instance of the use of this technique in Escherichia coli was published in 1989 by Hamilton, et al. [2] In this experiment, two sequential recombinations were used to delete the gene. This work established the feasibility of removing or replacing a functional gene in bacteria.
For altering moss genes in a targeted way, the DNA-construct needs to be incubated together with moss protoplasts and with polyethylene glycol (PEG). Because mosses are haploid organisms, the regenerating moss filaments (protonemata) can be directly assayed for gene targeting within six weeks when utilizing PCR methods.
Reported cases of dengue in the Americas nearly tripled to a record high of over 12.6 million this year, including 21,000 severe cases and over 7,700 deaths, the Pan American Health Organization ...
Shares of American Express (NYSE: AXP) stock shot up 58.4% in 2024, according to data from S&P Global Market Intelligence. The credit card and banking giant had close to everything working in its ...
Democratic Rep. Nancy Pelosi, 84, underwent a successful hip replacement surgery after falling while in Luxembourg with a congressional delegation, her office said Saturday. "Earlier this morning ...
The number of clones that constitute a genomic library depends on (1) the size of the genome in question and (2) the insert size tolerated by the particular cloning vector system. For most practical purposes, the tissue source of the genomic DNA is unimportant because each cell of the body contains virtually identical DNA (with some exceptions).