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The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. In a more strict sense, the term vital staining has a meaning contrasting with supravital staining.
Thus a supravital stain may have a greater toxicity, as only a few cells need to survive it a short while. The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive ...
In anatomy the term reticuloendothelial system (abbreviated RES), often associated nowadays with the mononuclear phagocyte system (MPS), was employed by the beginning of the 20th century to denote a system of specialised cells that effectively clear colloidal vital stains (so called because they stain living cells) from the blood circulation.
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. [2] The indicator Janus Green B changes colour according to the amount of oxygen present. [3] When oxygen is present, the indicator oxidizes to a blue colour.
Fluorescent stains are, however, phototoxic, invasive and bleach when observed. This limits their use when observing living cells over extended periods of time. Non-invasive phase-contrast techniques are therefore often used as a vital complement to fluorescent microscopy in live-cell imaging applications.
Once assembled, the Foldscope is about the size of a bookmark. The Foldscope weighs 8 grams [citation needed] and comes in a kit with a lens that magnifies 140X. The kit also includes magnets that can be stuck onto the Foldscope to attach it to a smartphone, allowing the user to take pictures of the magnification. [2]
Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology.It stains lysosomes red. [1] It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods.
It is a supravital stain. [2] It is closely related to methylene blue, an older stain in wide use. Safety. New methylene blue is toxic. Skin contact or inhalation ...