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Litmus paper after being used. The main use of litmus is to test whether a solution is acidic or basic, as blue litmus paper turns red under acidic conditions, and red litmus paper turns blue under basic or alkaline conditions, with the color change occurring over the pH range 4.5–8.3 at 25 °C (77 °F). Neutral litmus paper is purple. [2]
If the test solution is of a dark colour, it is preferable to use a paper universal indicator, such as Hydrion paper. Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. This mixture is important because each component loses or gains protons ...
The word litmus is literally from 'colored moss' in Old Norse (see Litr). The color changes between red in acid solutions and blue in alkalis. The term 'litmus test' has become a widely used metaphor for any test that purports to distinguish authoritatively between alternatives. Hydrangea macrophylla flowers can change color depending on soil ...
Bromothymol blue acts as a weak acid in a solution. It can thus be in protonated or deprotonated form, appearing yellow or blue, respectively. It is bright aquamarine by itself, and greenish-blue in a neutral solution. The deprotonation of the neutral form results in a highly conjugated structure, accounting for the difference in color. An ...
The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis . Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream.
Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered p K a results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5).
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