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The enzyme-linked immunosorbent assay or ELISA is a diagnostic method for quantitatively or semi-quantitatively determining protein concentrations from blood plasma, serum or cell/tissue extracts in a multi-well plate format (usually 96-wells per plate). Broadly, proteins in solution are absorbed to ELISA plates.
Here, I redesigned the 96-well plate figure working from an overlay of a 96-well plate manufacturer technical drawing, and the official SLAS/ANSI 96-well plate size standards. Designed in Inkscape. 16:42, 29 May 2020: 672 × 452 (70 KB) FlowerFaerie087: Minor edit for consistent, smooth image: 15:43, 29 May 2020: 672 × 452 (76 KB) FlowerFaerie087
The well position is also standardized, but only for 96- , 384-, and 1536-well plates. These are generally well followed by manufacturers: Well Positions [16] [17] 96-well plates have a 9 mm well-to-well spacing, 384-wells a 4.5 mm spacing, and 1536-wells a 2.25 mm spacing. A notable characteristic is that the well array is symmetrical when the ...
Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery , [ 1 ] bioassay validation, quality control and manufacturing processes in the pharmaceutical and ...
A robot arm handles an assay plate. The key labware or testing vessel of HTS is the microtiter plate, which is a small container, usually disposable and made of plastic, that features a grid of small, open divots called wells. In general, microplates for HTS have either 96, 192, 384, 1536, 3456 or 6144 wells.
One common method is to use low cell adhesion plates, typically a 96 well plate, to mass-produce spheroid cultures, where the aggregates form in the rounded bottom of the cell plates. [36] [42] Spheroids can also be cultured using the hanging drop method [43] involving forming cell aggregates in drops that hang from the surface of a cell plate ...
If the cells do not have high intracellular potassium and if intracellular sodium is low, then (1) the cell membrane may not be intact, and/or (2) the sodium-potassium pump may not be operating well. [6] [7] Flow cytometry using 7-Aminoactinomycin D (7-AAD), wherein a lower signal indicates viable cells. Therefore, this case shows good ...
A high throughput assay can be either an endpoint or a kinetic assay usually done on an automated platform in 96-, 384- or 1536-well microplate formats (High Throughput Screening). Such assays are able to test large number of compounds or analytes or make functional biological readouts in response to a stimuli and/or compounds being tested. [6]