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Primer extension is a technique whereby the 5' ends of RNA can be mapped - that is, they can be sequenced and properly identified. Primer extension can be used to determine the start site of transcription (the end site cannot be determined by this method) by which its sequence is known.
A house plan [1] is a set of construction or working drawings (sometimes called blueprints) that define all the construction specifications of a residential house such as the dimensions, materials, layouts, installation methods and techniques.
Benjamin Stephenson House's courtyard formed by an ell. In architecture, an ell is a wing of a building perpendicular (at a right angle) to the length of the main portion (main range). [1] It takes its name from the shape of the letter L. Ells are often additions to a building.
In the method, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid to form a duplex, with the primer’s terminal 3’-end directly adjacent to the nucleotide base to be identified. Using a DNA polymerase, the oligonucleotide primer is enzymatically extended by a single base in the presence of all four ...
The toeprinting assay, also known as the primer extension inhibition assay, [1] is a method used in molecular biology that allows one to examine the interactions between messenger RNA and ribosomes or RNA-binding proteins. [2] It is different from the more commonly used DNA footprinting assay. The toeprinting assay has been utilized to examine ...
A storey plan (the red floor would be the 5th in North American convention, or 4th in the European convention) A storey (Commonwealth English) [1] or story (American English; see spelling differences), [2] is any level part of a building with a floor that could be used by people (for living, work, storage, recreation, etc.).
Floor plan of a basic central-passage house. The central-passage house , also known variously as central hall plan house , center-hall house , hall-passage-parlor house , Williamsburg cottage , and Tidewater-type cottage , was a vernacular , or folk form, house type from the colonial period onward into the 19th century in the United States .
The Yeoman's House, Bignor, Sussex, a three-bay Wealden hall house. The hall house is a type of vernacular house traditional in many parts of England, Wales, Ireland and lowland Scotland, as well as northern Europe, during the Middle Ages, centring on a hall. Usually timber-framed, some high status examples were built in stone.