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A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision , from single piece glass pipettes to more complex adjustable or ...
Micropipette in action. Piston-driven air displacement pipettes are a type of micropipette, which are tools to handle volumes of liquid in the microliter scale. They are more commonly used in biology and biochemistry, and less commonly in chemistry; the equipment is susceptible to damage from many organic solvents.
They are used in the laboratory and also to dispense small amounts of liquid medicines. A very common use was to dispense eye drops into the eye. The commonly recognized form is a glass tube tapered to a narrow point (a pipette ) and fitted with a rubber bulb at the top, although many styles of both plastic and glass droppers exist.
Typical volumes are 1, 2, 5, 10, 20, 25, 50 and 100 mL. Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration. ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. The tolerance depends on the size: a 0.5-mL ...
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette.The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: [1] as it reduces the risk of splashing, foam or bubble formation.
The standard forward pipetting technique is used to dispense and mix a sample or reagent into another liquid Once a suitable tip is attached to the pipette, press its knob to the first stop. Dip the tip into the solution to a certain depth according to the volume set.
The standard technique for handling a graduated pipette is to hold the pipette tip dipped in the solution without touching the bottom of the beaker. Then use a propipetter, a pipette bulb, or rubber bulb, to draw the liquid into the pipette. The effective way to control the volume of the solution is to use one's forefinger. [2]
The Micropipette developed by Heinrich Schnitger, which is often referred to as "Eppendorf pipette" in laboratory usage, [citation needed] counts today in various designs as standard almost every biomedical laboratories. For biologists, biochemists, biology laboratory technicians, medical-technical assistants and similar professions handling of ...