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Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.
Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed. In animal tissue culture, cells may be grown as two-dimensional monolayers (conventional culture) or within fibrous ...
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is the most popular plant growth medium used in the laboratories worldwide for cultivation of plant cell culture on agar. MS0 was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS ...
The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer
Cell culture in a special tissue culture dish. Cell culture can be an alternative to animal use in some cases. For example, cultured cells have been developed to create monoclonal antibodies; prior to this, production required animals to undergo a procedure likely to cause pain and distress. [7]
Plant breeding can be performed using many different techniques, ranging from the selection of the most desirable plants for propagation, to methods that make use of knowledge of genetics and chromosomes, to more complex molecular techniques. Genes in a plant are what determine what type of qualitative or quantitative traits it will have.
Agricultural biotechnology, also known as agritech, is an area of agricultural science involving the use of scientific tools and techniques, including genetic engineering, molecular markers, molecular diagnostics, vaccines, and tissue culture, to modify living organisms: plants, animals, and microorganisms. [1]