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Virus quantification is counting or calculating the number of virus particles (virions) in a sample to determine the virus concentration. It is used in both research and development (R&D) in academic and commercial laboratories as well as in production situations where the quantity of virus at various steps is an important variable that must be monitored.
(A) When the host cell is only infected by a giant virus, the latter establishes a cytoplasmic virus factory to replicate and generates new virions, and the host cell is most likely lysed at the end of its replication cycle. (B) When the host cell is co-infected with a giant virus and its virophage, the latter parasitizes the giant virus factory.
A 2010 review study by Puren et al. [2] categorizes viral load testing into three types: (1) nucleic acid amplification based tests (NATs or NAATs) commercially available in the United States with Food and Drug Administration (FDA) approval, or on the market in the European Economic Area (EEA) with the CE marking; (2) "Home–brew" or in-house NATs; (3) non-nucleic acid-based test.
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The phi X 174 (or ΦX174) bacteriophage is a single-stranded DNA virus that infects Escherichia coli. This virus was isolated in 1935 by Nicolas Bulgakov [1] in Félix d'Hérelle's laboratory at the Pasteur Institute, from samples collected in Paris sewers. Its characterization and the study of its replication mechanism were carried out from ...
180 nm – typical length of the rabies virus; 200 nm – typical size of a Mycoplasma bacterium, among the smallest bacteria; 300 nm – greatest particle size that can fit through a HEPA (high efficiency particulate air) filter (N100 removes up to 99.97% at 300 nm, N95 removes up to 95% at 300 nm) [85] 300–400 nm – near ultraviolet wavelength
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The virus particle is enveloped and spherical, ovoid or variable in shape, with a diameter generally in the range 80–120 nm. [4] [13] [14] The virus particle contains around ten ribosome-like granules, a feature of arenaviruses. [4] It bears surface projections variously described as 4–5 nm long [4] and 10–14 nm in length and 4–6 nm in ...
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