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Cryopreservation of ovarian tissue is of interest to women who want fertility preservation beyond the natural limit, or whose reproductive potential is threatened by cancer therapy, [1] for example in hematologic malignancies or breast cancer. [2]
The same study found that, of 65 patients referred to the program, 28% declined to undergo embryo, oocyte, or tissue cryopreservation. 9% were found not to be eligible for medical reasons. Of the remaining 41 patients, 85% chose to cryopreserve embryos, 10% chose to cryopreserve oocytes, and 5% chose to undergo ovarian tissue freezing. [9]
Under ultrasound guidance, the operator inserts a 16.5 gauge × 11.8″ (1.6 mm × 300 mm outer diameter) needle through the vaginal wall and into an ovarian follicle, taking care not to injure nearby organs and blood vessels. The other end of the needle is attached to a suction device.
An artificial ovary is a potential fertility preservation treatment that aims to mimic the function of the natural ovary. Conventional fertility preservation for females involves oocyte cryopreservation or ovarian tissue cryopreservation. However, there are drawbacks to these treatments.
Oocyte cryopreservation is a procedure to preserve a woman's eggs . The technique is often used to delay pregnancy . At the time pregnancy is desired, the eggs can be thawed, fertilized, and transferred to the uterus as embryos .
Cryopreservation or cryoconservation is a process where biological material - cells, tissues, or organs - are frozen to preserve the material for an extended period of time. [1] At low temperatures (typically −80 °C (−112 °F) or −196 °C (−321 °F) using liquid nitrogen ) any cell metabolism which might cause damage to the biological ...
At least six major areas of cryobiology can be identified: 1) study of cold-adaptation of microorganisms, plants (cold hardiness), and animals, both invertebrates and vertebrates (including hibernation), 2) cryopreservation of cells, tissues, gametes, and embryos of animal and human origin for (medical) purposes of long-term storage by cooling to temperatures below the freezing point of water.
The cryopreservation of embryos was first successfully attempted in 1984 in the case of Zoe Leyland, the first baby to be born from a frozen embryo. [16] In Zoe's case, the embryo had been frozen for two months, but since the inception of the practice of cryopreservation after successful IVF, embryos have successfully survived in ...