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The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus. [1] [2] The serum sample or solution of antibody to be tested is diluted and mixed with a viral suspension. This is incubated to allow the antibody to react with the virus. This is poured over a confluent monolayer of host cells.
However, serum therapy is today still used as the first line of defence during an outbreak as it can relatively quickly obtained. [19] [20] Serum therapy was shown to reduce mortality in patients during the 2009 swine flu pandemic [21] and the Western African Ebola virus epidemic. [22] It is also being tested as possible treatment for COVID-19.
This technique makes many copies of the virus genome using virus-specific probes. Variations of PCR such as nested reverse transcriptase PCR and real time PCR can also be used to determine viral loads in patient serum. This is often used to monitor treatment success in HIV cases.
The complement fixation test is an immunological medical test that can be used to detect the presence of either specific antibody or specific antigen in a patient's serum, based on whether complement fixation occurs. It was widely used to diagnose infections, particularly with microbes that are not easily detected by culture methods, and in ...
Also sometimes called the Norwalk virus or winter vomiting bug, norovirus is an extremely contagious virus. It is a common cause of illness outbreaks on cruise ships, where more than 90% of ...
Virus quantification is counting or calculating the number of virus particles (virions) in a sample to determine the virus concentration. It is used in both research and development (R&D) in academic and commercial laboratories as well as in production situations where the quantity of virus at various steps is an important variable that must be monitored.
By Joseph Ax, Helen Coster and James Oliphant (Reuters) - U.S. President-elect Donald Trump's vow to impose tariffs on China and Mexico unless they stem the flow of fentanyl and migrants across ...
Reporter virus particles (RVPs) are replication-incompetent virus particles engineered to express one or more reporter genes upon infecting susceptible cells. [ 1 ] [ 2 ] [ 3 ] Since the RVP genome lacks genes essential for viral replication, RVPs are capable of only a single round of infection.
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