enow.com Web Search

  1. Ad

    related to: dna fragmentation assay protocol for dogs youtube

Search results

  1. Results from the WOW.Com Content Network
  2. TUNEL assay - Wikipedia

    en.wikipedia.org/wiki/TUNEL_assay

    The fluorochrome-based TUNEL assay applicable for flow cytometry, combining the detection of DNA strand breaks with respect to the cell cycle-phase position, was originally developed by Gorczyca et al. [4] Concurrently, the avidin-peroxidase labeling assay applicable for light absorption microscope was described by Gavrieli et al. [5] Since 1992 the TUNEL has become one of the main methods for ...

  3. Restriction digest - Wikipedia

    en.wikipedia.org/wiki/Restriction_digest

    Restriction digest is most commonly used as part of the process of the molecular cloning of DNA fragment into a vector (such as a cloning vector or an expression vector).The vector typically contains a multiple cloning site where many restriction site may be found, and a foreign piece of DNA may be inserted into the vector by first cutting the restriction sites in the vector as well the DNA ...

  4. DNA fragmentation - Wikipedia

    en.wikipedia.org/wiki/DNA_fragmentation

    Apoptotic DNA fragmentation is a natural fragmentation that cells perform in apoptosis (programmed cell death). DNA fragmentation is a biochemical hallmark of apoptosis.In dying cells, DNA is cleaved by an endonuclease that fragments the chromatin into nucleosomal units, which are multiples of about 180-bp oligomers and appear as a DNA ladder when run on an agarose gel. [8]

  5. Apoptotic DNA fragmentation - Wikipedia

    en.wikipedia.org/wiki/Apoptotic_DNA_fragmentation

    The apoptotic DNA fragmentation is being used as a marker of apoptosis and for identification of apoptotic cells either via the DNA laddering assay, [2] the TUNEL assay, [3] [4] or the by detection of cells with fractional DNA content ("sub G 1 cells") on DNA content frequency histograms e.g. as in the Nicoletti assay.

  6. Sperm Chromatin Structure Assay - Wikipedia

    en.wikipedia.org/.../Sperm_Chromatin_Structure_Assay

    Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA fragmentation. [1] First described by Evenson in 1980, the assay is a flow cytometric test that detects the vulnerability of sperm DNA to acid-induced denaturation DNA in situ. [2]

  7. DNase footprinting assay - Wikipedia

    en.wikipedia.org/wiki/DNase_footprinting_assay

    The lane labelled "control" is for quality control purposes and contains the DNA fragment but not treated with DNase I. A DNase footprinting assay [1] is a DNA footprinting technique from molecular biology/biochemistry that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic ...

  8. DNA laddering - Wikipedia

    en.wikipedia.org/wiki/DNA_laddering

    DNA laddering (left) visualised in an agarose gel by ethidium bromide staining. A 1 kb marker (middle) and control DNA (right) are included.. DNA laddering is a feature that can be observed when DNA fragments, resulting from Apoptosis DNA fragmentation are visualized after separation by gel electrophoresis the first described in 1980 by Andrew Wyllie at the University Edinburgh medical school ...

  9. Comet assay - Wikipedia

    en.wikipedia.org/wiki/Comet_assay

    The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. It was first developed by Östling & Johansson in 1984 and later modified by Singh et al. in 1988. [ 1 ]

  1. Ad

    related to: dna fragmentation assay protocol for dogs youtube