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Sonication is the act of applying sound energy to agitate particles in a sample, for various purposes such as the extraction of multiple compounds from plants, microalgae and seaweeds. [1] Ultrasonic frequencies (> 20 kHz) are usually used, leading to the process also being known as ultrasonication or ultra-sonication .
Cell lysis is used in laboratories to break open cells and purify or further study their contents. Lysis in the laboratory may be affected by enzymes or detergents or other chaotropic agents . Mechanical disruption of cell membranes, as by repeated freezing and thawing, sonication , pressure, or filtration may also be referred to as lysis.
C4 plants use a modified Calvin cycle in which they separate Ribulose-1,5-bisphosphate carboxylase oxygenase (RuBisCO) from atmospheric oxygen, fixing carbon in their mesophyll cells and using oxaloacetate and malate to ferry the fixed carbon to RuBisCO and the rest of the Calvin cycle enzymes isolated in the bundle-sheath cells.
Some shade-loving plants (sciophytes) produce such low levels of oxygen during photosynthesis that they use all of it themselves instead of releasing it to the atmosphere. [12] Although there are some differences between oxygenic photosynthesis in plants, algae, and cyanobacteria, the overall process is quite similar in these organisms.
The evolution of oxygen during the light-dependent steps in photosynthesis (Hill reaction) was proposed and proven by British biochemist Robin Hill. He demonstrated that isolated chloroplasts would make oxygen (O 2) but not fix carbon dioxide (CO 2). This is evidence that the light and dark reactions occur at different sites within the cell. [1 ...
The first ideas about light being used in photosynthesis were proposed by Jan IngenHousz in 1779 [9] who recognized it was sunlight falling on plants that was required, although Joseph Priestley had noted the production of oxygen without the association with light in 1772. [10]
Cellular respiration is a vital process that occurs in the cells of all [[plants and some bacteria ]]. [2] [better source needed] Respiration can be either aerobic, requiring oxygen, or anaerobic; some organisms can switch between aerobic and anaerobic respiration. [3] [better source needed]
These plants differ from C3 plants because CO 2 is initially converted to a four-carbon molecule, malate, which is shuttled to bundle sheath cells, released back as CO 2 and only then enters the Calvin Cycle. In contrast, C3 plants directly perform the Calvin Cycle in mesophyll cells, without making use of a CO 2 concentration method. Malate ...