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  2. Disk diffusion test - Wikipedia

    en.wikipedia.org/wiki/Disk_diffusion_test

    The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture-based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the susceptibility ...

  3. Mueller–Hinton agar - Wikipedia

    en.wikipedia.org/wiki/Mueller–Hinton_agar

    Mueller Hinton agar is commonly used in the disk diffusion method, which is a simple and widely used method for testing the susceptibility of bacterial isolates to antibiotics. In this method, small disks impregnated with different antibiotics are placed on the surface of the agar, and the zone of inhibition around each disk is measured to ...

  4. Agar - Wikipedia

    en.wikipedia.org/wiki/Agar

    Green tea-flavored yōkan, a popular Japanese red bean jelly made from agar A blood agar plate used to culture bacteria and diagnose infection. Agar (/ ˈ eɪ ɡ ɑːr / or / ˈ ɑː ɡ ər /), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from "ogonori" and "tengusa".

  5. Agar plate - Wikipedia

    en.wikipedia.org/wiki/Agar_plate

    Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.

  6. Agar dilution - Wikipedia

    en.wikipedia.org/wiki/Agar_dilution

    Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria.

  7. Antibiotic sensitivity testing - Wikipedia

    en.wikipedia.org/wiki/Antibiotic_sensitivity_testing

    The "gutter method" that he developed was a diffusion method, involving an antibiotic that was diffused through a gutter made of agar. [25] In the 1940s, multiple investigators, including Pope, Foster and Woodruff, Vincent and Vincent used paper discs instead. [25] All these methods involve testing only susceptibility to penicillin. [25]

  8. Microbiological culture - Wikipedia

    en.wikipedia.org/wiki/Microbiological_culture

    After the desired level of growth is achieved, agar plates can be stored upside down in a refrigerator for an extended period of time to keep bacteria for future experiments. There are a variety of additives that can be added to agar before it is poured into a plate and allowed to solidify. Some types of bacteria can only grow in the presence ...

  9. Potato dextrose agar - Wikipedia

    en.wikipedia.org/wiki/Potato_dextrose_agar

    Aspergillus sp. growing in potato dextrose agar Potato dextrose agar (BAM Media M127) and potato dextrose broth are common microbiological growth media made from potato infusion and dextrose. Potato dextrose agar (abbreviated "PDA") is the most widely used medium for growing fungi and bacteria. PDA has the capability to culture various bacteria and fungi found in the soil. This agar can be ...

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