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Cholera toxin mechanism. Cholera toxin (also known as choleragen and sometimes abbreviated to CTX, Ctx or CT) is an AB5 multimeric protein complex secreted by the bacterium Vibrio cholerae. [1] [2] CTX is responsible for the massive, watery diarrhea characteristic of cholera infection. [3] It is a member of the heat-labile enterotoxin family.
After their B subunit binds to receptors on the cell surface, the toxin is enveloped by the cell and transported inside either through clathrin-dependent endocytosis or clathrin-independent endocytosis. [21] The mechanism pathways for the four AB5 toxins: cholera toxin, pertussis toxin, shiga toxin, and subtilase cytotoxin.
During infection, V. cholerae secretes cholera toxin (CT), a protein that causes profuse, watery diarrhea (known as "rice-water stool"). [33] [5] This cholera toxin contains 5 B subunits that plays a role in attaching to the intestinal epithelial cells and 1 A subunit that plays a role in toxin activity.
This toxin has the ability to disrupt electrolyte balance in intestinal epithelial cells which can lead to issues including severe diarrhea, which is known to be a common symptom of this toxin. [15] In addition to the cholera toxin, there are other virulence factors such as surface adhesins, which are essential in helping the bacteria to adhere ...
The cholera toxin (CTX or CT) is an oligomeric complex made up of six protein subunits: a single copy of the A subunit (part A), and five copies of the B subunit (part B), connected by a disulfide bond. The five B subunits form a five-membered ring that binds to GM1 gangliosides on the surface of the
The first of these is accessory cholera enterotoxin (Ace). Ace is currently thought to be a minor coat protein of virion stage CTXφ, though the process by which the toxin could be released from the protein coat has not yet been identified. The second non-CT toxin encoded within the CTXφ genome is zonula occludens toxin (Zot). Zot, though ...
V. cholerae is the most common pathogen that causes cholera. The gold standard for detecting cholera is through cultures of stool samples or rectal swabs. Identification is then done through microscopy or by agglutination of antibodies. [25] Cultures are done in thiosulfate citrate bile-salts sucrose agar. V cholerae will form yellow colonies. [26]
Nonetheless, Koch was already aware that the causative agent of cholera, Vibrio cholerae, could be found in both sick and healthy people, invalidating his first postulate. [ 6 ] [ 9 ] Since the 1950s, Koch's postulates have been treated as obsolete for epidemiology research, but they are still taught to emphasize historical approaches to ...