Ad
related to: how to improve chromatography resultssartorius.com has been visited by 10K+ users in the past month
- Welcome to Sartorius
Your trusted partner.
For the biopharmaceutical industry.
- Fluid Management
The pioneer in single-use.
Find your product by process step.
- Explore the New Portfolio
Lab and Bioprocess Businesses
Chromatography, TFF & Microcarrier
- Chromatography Over Time
The history of chromatography.
Read the article on our blog.
- Welcome to Sartorius
Search results
Results from the WOW.Com Content Network
The fundamental resolution equation is used in chromatography to help relate adjustable chromatographic parameters to resolution, and is as follows: . R s = [N 1/2 /4][(α-1)/α][k 2 '/(1+k 2 ')], where
Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds.
Sealed chromatography cartridges or columns work similarly except the sample and buffer is pumped into and through the resin by an external device such as a liquid chromatographic (LC) system, also requiring collection and monitoring of several fractions. Even though this method is often semi-automated, using chromatography cartridges is ...
The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography. [8] The procedure begins with development of a sample loaded plate with first solvent. After removing it, the plate is rotated 90° and developed with a second solvent.
In the calibration curve that uses the internal standard, the y-axis is the ratio of the nickel signal to the yttrium signal. This ratio is unaffected by uncertainty in the nickel measurements, as it should affect the yttrium measurements in the same way. This results in a higher R 2, 0.9993.
Usually a reversed phase liquid chromatography is used, in which the peptides stay bound to the chromatography matrix whereas the salts are removed by washing. The peptides can be eluted from the matrix by the use of a small volume of a solution containing a large portion of organic solvent, which results in the reduction of the final volume of ...
A post-column oxidation-reduction reactor is a chemical reactor that performs derivatization to improve the quantitative measurement of organic analytes.It is used in gas chromatography (GC), after the column and before a flame ionization detector (FID), to make the response factor of the detector uniform for all carbon-based species.
The Standard addition method, often used in analytical chemistry, quantifies the analyte present in an unknown.This method is useful for analyzing complex samples where a matrix effect interferes with the analyte signal.
Ad
related to: how to improve chromatography resultssartorius.com has been visited by 10K+ users in the past month