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  2. Ribotyping - Wikipedia

    en.wikipedia.org/wiki/Ribotyping

    Ribotyping is a molecular technique for bacterial identification and characterization that uses information from rRNA-based phylogenetic analyses. [1] It is a rapid and specific method widely used in clinical diagnostics and analysis of microbial communities in food, water, and beverages.

  3. Terminal restriction fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Terminal_restriction...

    Following the restriction reaction, the mixture of fragments is separated using either capillary or polyacrylamide electrophoresis in a DNA sequencer and the sizes of the different terminal fragments are determined by the fluorescence detector. Because the excised mixture of amplicons is analyzed in a sequencer, only the terminal fragments (i.e ...

  4. Ribosomal RNA - Wikipedia

    en.wikipedia.org/wiki/Ribosomal_RNA

    In prokaryotes a small 30S ribosomal subunit contains the 16S ribosomal RNA. The large 50S ribosomal subunit contains two rRNA species (the 5S and 23S ribosomal RNAs). Therefore it can be deduced that in both bacteria and archaea there is one rRNA gene that codes for all three rRNA types :16S, 23S and 5S. [27] Bacterial 16S ribosomal RNA, 23S ...

  5. Community fingerprinting - Wikipedia

    en.wikipedia.org/wiki/Community_Fingerprinting

    The fragments can be run out on a gel (RISA), or the fluorescent primers can be translated into peaks in abundance of the different fragments lengths on an electropherogram (ARISA). [16] [17] Figure 3. The microbial fingerprinting technique called (Automated) Ribosomal Intergenic Spacer Analysis.

  6. Gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis

    Gel electrophoresis is an electrophoresis method for separation and analysis of biomacromolecules (DNA, RNA, proteins, etc.) and their fragments, based on their size and charge through a gel.

  7. Signal recognition particle RNA - Wikipedia

    en.wikipedia.org/.../Signal_recognition_particle_RNA

    Most bacterial SRPs are composed of SRP RNA and SRP54 (also named Ffh for "Fifty-four homolog"). The Archaeal SRP contains proteins SRP54 and SRP19. In eukaryotes, the SRP RNA combines with the imported SRP proteins SRP9/14, SRP19, and SRP68/72 in a region of the nucleolus. This pre-SRP is transported to the cytosol where it binds to protein ...

  8. RNA - Wikipedia

    en.wikipedia.org/wiki/RNA

    In the cytoplasm, ribosomal RNA and protein combine to form a nucleoprotein called a ribosome. The ribosome binds mRNA and carries out protein synthesis. Several ribosomes may be attached to a single mRNA at any time. [27] Nearly all the RNA found in a typical eukaryotic cell is rRNA. Transfer-messenger RNA (tmRNA) is found in many bacteria and ...

  9. Northern blot - Wikipedia

    en.wikipedia.org/wiki/Northern_blot

    An RNA ladder is often run alongside the samples on an electrophoresis gel to observe the size of fragments obtained but in total RNA samples the ribosomal subunits can act as size markers. [11] Since the large ribosomal subunit is 28S (approximately 5kb) and the small ribosomal subunit is 18S (approximately 2kb) two prominent bands appear on ...

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