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A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the rearrangement is present. Fluorescence in situ hybridization ( FISH) is a molecular cytogenetic ...
Q-FISH. Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled ( Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and ...
OECD Guidelines for the Testing of Chemicals ( OECD TG) are a set of internationally accepted specifications for the testing of chemicals decided on by the Organisation for Economic Co-operation and Development (OECD). They were first published in 1981. They are split into five sections: Section 1: Physical Chemical Properties.
Fluorescein diacetate hydrolysis. Fluorescein diacetate ( FDA) hydrolysis assays can be used to measure the enzyme activity of microbes in a sample. A bright yellow-green glow is produced and is strongest when enzymatic activity is greatest. This can be quantified using a spectrofluorometer or a spectrophotometer .
Squalidae, more commonly known as dogfish, dog sharks, or spiny dogfish, [ 3] are one of several families of sharks categorized under Squaliformes, making it the second largest order of sharks, numbering 119 species across 7 families. [ 4] Having earned their name after a group of fishermen reportedly observed the species chasing down smaller ...
Oyster toadfish. The oyster toadfish ( Opsanus tau ), also known as the oyster toad, ugly toad, oyster cracker, oyster catcher, and bar dog, is a Northwest Atlantic species of fish of the family Batrachoididae. The maximum length of this toadfish is 43.2 cm (17 in), but they infrequently surpass 38 cm (15 in).
It affects over 50 species of freshwater and marine fish in the northern hemisphere. 1. This flatfish Limanda limanda has an outgrowth called a xenoma. It is caused by a microsporidian fungal parasite in its intestines. 2. Like humans and other animals, fish suffer from diseases and parasites.
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue ( in situ) or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH ...