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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
One of the most important properties of microscope objectives is their magnification.The magnification typically ranges from 4× to 100×. It is combined with the magnification of the eyepiece to determine the overall magnification of the microscope; a 4× objective with a 10× eyepiece produces an image that is 40 times the size of the object.
A 4Pi microscope is a laser-scanning fluorescence microscope with an improved axial resolution. The typical value of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy .
1957: Marvin Minsky, a professor at MIT, invents the confocal microscope, an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. This technology is a predecessor to today's widely used confocal laser scanning microscope.
Stepwise magnification by 6% per frame into a 39-megapixel image. In the final frame, at about 170x, an image of a bystander is seen reflected in the man's cornea. Magnification is the process of enlarging the apparent size, not physical size, of something. This enlargement is quantified by a size ratio called optical magnification.
The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy. [1] The term MOLE (molecular optics laser examiner) is used to refer to the Raman-based microprobe. [ 1 ] The technique used is named after C. V. Raman , who discovered the scattering properties in liquids.
All optical microscopes are diffraction-limited because of the wave nature of light. Current research focuses on techniques to go beyond this limit known as the Rayleigh criterion . The use of SIL can achieve spatial resolution better than the diffraction limit in air, for both far-field imaging [ 3 ] [ 4 ] and near-field imaging.
Laser scanning is the controlled deflection of laser beams, visible or invisible. [1] Scanned laser beams are used in some 3-D printers, in rapid prototyping, in machines for material processing, in laser engraving machines, in ophthalmological laser systems for the treatment of presbyopia, in confocal microscopy, in laser printers, in laser shows, in Laser TV, and in barcode scanners.
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