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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
A buffer solution is a solution where the pH does ... (PBS) at pH 7.4. In industry, buffering agents are used in fermentation processes ... monopotassium phosphate, ...
Dulbecco's phosphate-buffered saline, a buffer solution used in biological research DPBS (CONFIG.SYS directive) , a configuration directive in DOS Topics referred to by the same term
In the case of a phosphate buffer, substantial quantities of weak acid and conjugate weak base (H 2 PO 4 – and HPO 4 2–) can accept or donate protons accordingly in order to conserve intracellular pH: [13] [14] OH – + H 2 PO 4 – ⇌ H 2 O + HPO 4 2– H + + HPO 4 2– ⇌ H 2 PO 4 –
Tris-buffered saline (TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range. The conjugate acid of Tris has a pK a of 8.07 at 25 °C.
For isolating nuclei, components include Dulbecco's phosphate-buffered saline (DPBS), trypsin or dispase (depending on cell type), trypan blue, hemocytometer, lysis buffer, and wash buffer To treat nuclei with M.CviPl, components include GpC Buffer, S-adenosylhomocysteine (SAM), M.CviPl, Sucrose, Nuclease-free water, and Stop buffer
After obtaining the umbilical cord from the donor, it is transported to the laboratory in a cold and sterile environment using a transfer buffer that contains phosphate buffered saline (PBS). Subsequently, the vessels should be eliminated from the umbilical cord tissue on a sterile plate with the aid of autoclaved scissors. The sections of the ...
Paraformaldehyde is not a fixative; it must be depolymerized to formaldehyde in solution. In cell culture, a typical formaldehyde fixing procedure would involve using a 4% formaldehyde solution in phosphate buffered saline (PBS) on ice for 10 minutes. In histology and pathology specimens preparation, usually, the fixation step is performed ...
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