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Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
Colorimetry is "the science and technology used to quantify and describe physically the human color perception". [1] It is similar to spectrophotometry , but is distinguished by its interest in reducing spectra to the physical correlates of color perception, most often the CIE 1931 XYZ color space tristimulus values and related quantities.
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
A camera or colorimeter is said to be colorimetric if it satisfies the Luther condition by Robert Luther (1868–1945) (also called the "Maxwell–Ives criterion"), [5] reducing observer metamerism color errors, if the product of the spectral responsivity of the photoreceptor and the spectral transmittance of the filters is a linear combination ...
Forensic colorimetry, or forensic color analysis, is the examination of specimen color for purposes of forensic investigation.Typical specimens involved in color analyses include pigments, dyes, or other objects that are distinguishable by their intrinsic color.
A colorimeter is a device used in colorimetry that measures the absorbance of particular wavelengths of light by a specific solution. [1] [2] It is commonly used to determine the concentration of a known solute in a given solution by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.
Determination of iron by a colorimetric method Determination of iron by a colorimetric method, top view. Nessler cylinders (also named color comparison cylinders or color comparing cylinders) are laboratory tubes with a fixed volume, made of glass with optically plane bottom. On the walls, there are marks of the nominal stroke volume (usually ...
Berthelot's reagent is an alkaline solution of phenol and hypochlorite, used in analytical chemistry.It is named after its inventor, Marcellin Berthelot.Ammonia reacts with Berthelot's reagent to form a blue product which is used in a colorimetric method for determining ammonia.