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Medical cryotherapy gun with liquid nitrogen. In medical applications, spray cans containing dimethyl ether [4] or tetrafluoroethane may also be used to freeze and destroy tissue, for removal of warts and skin tags, or other uses in cryosurgery. Liquified petroleum gas including propane and butane is sometimes used.
The temperature of liquid nitrogen can readily be reduced to its freezing point −210 °C (−346 °F; 63 K) by placing it in a vacuum chamber pumped by a vacuum pump. [2] Liquid nitrogen's efficiency as a coolant is limited by the fact that it boils immediately on contact with a warmer object, enveloping the object in an insulating layer of ...
First, the liquid nitrogen boils rapidly around the specimen forming a film of insulating N 2 gas that slows heat transfer to the cryogenic liquid, known as the Leidenfrost effect. Cooling rates can be improved by pumping the liquid nitrogen with a rotary vane vacuum pump for a few tens of seconds before plunging the specimen into it. This ...
Liquid N 2: Isoamyl acetate-79 Dry ice: Sulfur dioxide-82 Liquid N 2: Ethyl Acetate-84 Liquid N 2: n-Butanol-89 Liquid N 2: Hexane-94 Liquid N 2: Acetone-94 Liquid N 2: Toluene-95 Liquid N 2: Methanol-98 Liquid N 2: Cyclohexene-104 Liquid N 2: Isooctane-107 Liquid N 2: Ethyl iodide-109 Liquid N 2: Carbon disulfide-110 Liquid N 2: Butyl bromide ...
In vacuum applications, a cold trap is a device that condenses all vapors except the permanent gases (hydrogen, oxygen, and nitrogen) into a liquid or solid. [ 2 ] [ needs update ] The most common objective is to prevent vapors being evacuated from an experiment from entering a vacuum pump where they would condense and contaminate it.
This Wikipedia page provides a comprehensive list of boiling and freezing points for various solvents.
In this way, the cryoprotectant prevents actual freezing, and the solution maintains some flexibility in a glassy phase. Many cryoprotectants also function by forming hydrogen bonds with biological molecules as water molecules are displaced. Hydrogen bonding in aqueous solutions is important for proper protein and DNA function.
A tank of liquid nitrogen, used to supply a cryogenic freezer (for storing laboratory samples at a temperature of about −150 °C or −238 °F) Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), [18] is a technique where cells are cooled to around -196 °C over the course of several hours.
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