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Magnification is the ability to make small objects seem larger, such as making a microscopic organism visible. Resolution is the ability to distinguish two objects from each other. Light microscopy has limits to both its resolution and its magnification.
The main difference between magnification and resolution is their effect on image quality. Magnification enlarges the specimen, whereas resolution helps in improving the clarity.
With the help of proper illumination, a microscope can magnify a specimen and optically resolve fine detail. This introduction to microscopy will include an explanation of features and adjustments of a compound brightfield light microscope, which magnifies images using a two lens system.
They include information about the magnification of the image (for example, 600x) as well as a scale bar, which acts as a ruler and indicates the true size of the object. These conventions help others to make sense of the images. Explore the worlds within worlds of detail in the microscopic scale.
Resolution is intrinsically linked to the numerical aperture (NA) of a microscope’s optical components, like the objective lens, as well as the wavelength of light used. This article covers some of the history behind resolution concepts and explains each one using relatively simple terminology.
Resolution & Magnification. Magnification is how many times bigger the image of a specimen observed is in compared to the actual (real-life) size of the specimen. A light microscope has two types of lens: An eyepiece lens, which often has a magnification of x10. A series of (usually 3) objective lenses, each with a different magnification.
The resolution of an optical microscope is defined as the shortest distance between two points on a specimen that can still be distinguished by the observer or camera system as separate entities.