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Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar.
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis , [ 2 ] Francisella and Nocardia spp .
Hardy Diagnostics is an American company that manufactures and sells bacteriological culture media, reagents, automated microscope slide staining machines, and rapid identification kits for microbiological testing in clinical, research, and industrial laboratories. The company's culture media is useful in the detection of bacterial pathogens ...
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Granada agar is used for the primary isolation, identification and screening of β-hemolytic GBS from clinical specimens. [2] [4] This culture medium is selective for GBS, nevertheless other microorganisms (such as enterococci and yeasts), resistant to the selective agents used, can develop as colorless or white colonies. [2]
Lysine iron agar or LIA is a differential media used to distinguish bacteria that are able to decarboxylate lysine and/or produce hydrogen sulfide from those that cannot. [1] This test is particularly useful for distinguishing different Gram-negative bacilli —especially among the Enterobacteriaceae .
CLED agar (cystine–lactose–electrolyte-deficient agar or medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary microbes. It contains cystine and lactose and is electrolyte -deficient; the latter trait prevents the swarming of Proteus species.
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.