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The most common, conventional extraction process involves six steps: pre-treatment of the algal biomass, acid treatment, alkaline extraction, precipitation, bleaching, and drying. [4] Pre-treatments mainly aim at either breaking the cell wall to help extract the alginate, or removing other compounds and contaminants from the algae. [4]
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The template synthesis method uses a nanoporous membrane template composed of cylindrical pores of uniform diameter to make fibrils (solid nanofiber) and tubules (hollow nanofiber). [51] [52] This method can be used to prepare fibrils and tubules of many types of materials, including metals, semiconductors and electronically conductive polymers.
Calcium alginate is a water-insoluble, gelatinous, cream-coloured substance that can be created through the addition of aqueous calcium chloride to aqueous sodium alginate. Calcium alginate is also used for entrapment of enzymes and forming artificial seeds in plant tissue culture.
Adsorption on glass, alginate beads or matrix: Enzyme is attached to the outside of an inert material. In general, this method is the slowest among those listed here. As adsorption is not a chemical reaction , the active site of the immobilized enzyme may be blocked by the matrix or bead, greatly reducing the activity of the enzyme.
Laboratory-scale liquid-liquid extraction. Photograph of a separatory funnel in a laboratory scale extraction of 2 immiscible liquids: liquids are a diethyl ether upper phase, and a lower aqueous phase. Soxhlet extractor. Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix. The distribution ...
Extraction Profile for Different Types of Extraction. The extraction curve of % recovery against time can be used to elucidate the type of extraction occurring. Figure 4(a) shows a typical diffusion controlled curve. The extraction is initially rapid, until the concentration at the surface drops to zero, and the rate then becomes much slower.
Accelerated solvent extraction (ASE), also known as pressurized solvent extraction (PSE), is a method for extracting various chemicals from a complex solid or semisolid sample matrix. The process uses high temperature and pressure, which results in the extraction taking less time and requiring less solvent, and possibly also giving better ...