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The polymerase chain reaction is the most widely used method for in vitro DNA amplification for purposes of molecular biology and biomedical research. [1] This process involves the separation of the double-stranded DNA in high heat into single strands (the denaturation step, typically achieved at 95–97 °C), annealing of the primers to the single stranded DNA (the annealing step) and copying ...
This helicase was described as a "DNA unwinding enzyme" that is "found to denature DNA duplexes in an ATP-dependent reaction, without detectably degrading". [13] The first eukaryotic DNA helicase discovered was in 1978 in the lily plant. [ 14 ]
Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, is an enzyme that in humans is encoded by the WRN gene. WRN is a member of the RecQ Helicase family. [ 5 ] Helicase enzymes generally unwind and separate double-stranded DNA .
RecQ helicase is a family of helicase enzymes initially found in Escherichia coli [1] that has been shown to be important in genome maintenance. [2] [3] [4] They function through catalyzing the reaction ATP + H 2 O → ADP + P and thus driving the unwinding of paired DNA and translocating in the 3' to 5' direction.
Cdc6 binds to the ORC on DNA in an ATP-dependent manner, ... and the complex of CDC45-Mcm-GINS is the functional DNA helicase in eukaryotic cells. [64]
This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase.
ATP-dependent DNA helicase Q1 is an enzyme that in humans is encoded by the RECQL gene. [5] [6] [7] The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair.
DNA-dependent ATPase, abbreviated Dda and also known as Dda helicase and Dda DNA helicase, is the 439-amino acid 49,897-atomic mass unit protein coded by the Dda gene of the bacteriophage T4 phage, a virus that infects enterobacteria.