enow.com Web Search

Search results

  1. Results from the WOW.Com Content Network
  2. CRISPR gene editing - Wikipedia

    en.wikipedia.org/wiki/CRISPR_gene_editing

    CRISPR-Cas9 genome editing techniques have many potential applications. The use of the CRISPR-Cas9-gRNA complex for genome editing [10] was the AAAS's choice for Breakthrough of the Year in 2015. [11] Many bioethical concerns have been raised about the prospect of using CRISPR for germline editing, especially in human embryos. [12]

  3. Site-directed mutagenesis - Wikipedia

    en.wikipedia.org/wiki/Site-directed_mutagenesis

    Since 2013, the development of CRISPR-Cas9 technology has allowed for the efficient introduction of various mutations into the genome of a wide variety of organisms. The method does not require a transposon insertion site, leaves no marker, and its efficiency and simplicity has made it the preferred method for genome editing .

  4. Gene knockout - Wikipedia

    en.wikipedia.org/wiki/Gene_knockout

    The process of gene knockout with CRISPR involves three main steps: designing a guide RNA (gRNA) that targets a specific location in the genome, delivering the gRNA and a Cas9 enzyme (which acts as a molecular scissors) to the target cell, and then allowing the cell to repair the cut in the DNA.

  5. CRISPR interference - Wikipedia

    en.wikipedia.org/wiki/CRISPR_interference

    CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic cells. [1] It was first developed by Stanley Qi and colleagues in the laboratories of Wendell Lim , Adam Arkin, Jonathan Weissman , and Jennifer Doudna . [ 2 ]

  6. CRISPR activation - Wikipedia

    en.wikipedia.org/wiki/CRISPR_activation

    See: Guide RNA, CRISPR. Complementary base pairing between the sgRNA and genomic DNA allows targeting of Cas9 or dCas9. A small guide RNA (sgRNA), or gRNA is an RNA with around 20 nucleotides used to direct Cas9 or dCas9 to their targets. gRNAs contain two major regions of importance for CRISPR systems: the scaffold and spacer regions.

  7. CRISPR - Wikipedia

    en.wikipedia.org/wiki/CRISPR

    Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within living organisms.

  8. CRISPR RNA - Wikipedia

    en.wikipedia.org/wiki/CRISPR_RNA

    Type-II CRISPR systems [7] are characterized by the single signature nuclease Cas9. [8] In type-II CRISPR systems crRNA and tracrRNA (trans-activating CRISPR RNA) can form a complex known as the guide RNA or gRNA. [9] The crRNA within the gRNA is what matches up with the target sequence or protospacer after the PAM is found. Once the match is ...

  9. Off-target genome editing - Wikipedia

    en.wikipedia.org/wiki/Off-target_genome_editing

    While the Cas9 specificity is believed to be controlled by the 20nt sgRNA and PAM, off-target mutations are still prevalent and could occur with as many as 3-5 base pair mismatches (out of 20) between the sgRNA and the target DNA sequence. [25] [30] Furthermore, sgRNA secondary structures could also affect cleavage of on-target and off-target ...