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CRISPR gene editing technology in humans has the potential to cause profound social impacts, [112] such as in the long-term prevention of diseases in humans. [113] However, He's human experiments raised ethical concerns the effect are unknown on future generations. [112]
CRISPR-Cas9 genome editing techniques have many potential applications. The use of the CRISPR-Cas9-gRNA complex for genome editing [10] was the AAAS's choice for Breakthrough of the Year in 2015. [11] Many bioethical concerns have been raised about the prospect of using CRISPR for germline editing, especially in human embryos. [12]
Immunogenicity, off-target effects, mutations, delivery systems, and ethical issues are the main challenges that CRISPR technology faces. The safety concerns, ethical considerations, and the potential for misuse underscore the need for careful and responsible exploration of these technologies. [50] CRISPR-Cas9 technology offers so much on ...
Researchers have been able to manipulate large chunks of genetic code for almost 50 years. This newfound ability is called gene-editing, the tool is called CRISPR, and it’s being used worldwide ...
Problems arose such as off-target cleavage and the competitive recombination of the endogenous delta-globin with CRISPR/Cas9 led to unexpected mutation. The study results indicated that HBB repair in the embryos occurred preferentially through alternative pathways. In the end only 4 of the 54 zygotes carried the intended genetic information ...
Fast-paced developments in the CRISPR-Cas9 gene editing technology has increased both the concerns and relevance of this ethical controversy as it has become more popularly used. [19] [20] The scientific community recommends continued evaluation of risks and benefits of utilizing genetically modified organisms in everyday life. [21]
Off-target genome editing refers to nonspecific and unintended genetic modifications that can arise through the use of engineered nuclease technologies such as: clustered, regularly interspaced, short palindromic repeats ()-Cas9, transcription activator-like effector nucleases (), meganucleases, and zinc finger nucleases (ZFN). [1]
The Hwang affair, [1] or Hwang scandal, [2] or Hwanggate, [3] is a case of scientific misconduct and ethical issues surrounding a South Korean biologist, Hwang Woo-suk, who claimed to have created the first human embryonic stem cells by cloning in 2004.
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