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Deoxyribonuclease II (EC 3.1.22.1, DNase II, pancreatic DNase II, deoxyribonucleate 3'-nucleotidohydrolase, pancreatic DNase II, acid deoxyribonuclease, acid DNase) is an endonuclease that hydrolyzes phosphodiester linkages of deoxyribonucleotide in native and denatured DNA, yielding products with 3'-phosphates and 5'-hydroxyl ends, which occurs as a result of single-strand cleaving mechanism. [1]
DNase I contains four ion-binding pockets, and requires Ca 2+ and Mg 2+ for hydrolyzing double-stranded DNA. [5] Two of the sites strongly bind Ca 2+ while the other two coordinate Mg 2+ . Little has been published on the number and location of the Mg 2+ binding sites, although it has been proposed that Mg 2+ is located near the catalytic ...
Ribonuclease (commonly abbreviated RNase) is a type of nuclease that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC 2.7 (for the phosphorolytic enzymes) and 3.1 (for the hydrolytic enzymes) classes of enzymes.
In comparison, RNase A, a protein that catalyzes the same reaction, uses a coordinating histidine and lysine to act as a base to attack the phosphate backbone. [2] [clarification needed] Like many protein enzymes, metal binding is also critical to the function of many ribozymes. [9]
13423 Ensembl ENSG00000105612 ENSMUSG00000003812 UniProt O00115 P56542 RefSeq (mRNA) NM_001375 NM_010062 NM_001368295 NM_001368296 RefSeq (protein) NP_001366 NP_001366.1 NP_034192 NP_001355224 NP_001355225 Location (UCSC) Chr 19: 12.88 – 12.88 Mb n/a PubMed search Wikidata View/Edit Human View/Edit Mouse Deoxyribonuclease II, lysosomal is a protein that in humans is encoded by the DNASE2 ...
Depiction of the restriction enzyme (endonuclease) HindIII cleaving a double-stranded DNA molecule at a valid restriction site (5'–A|AGCTT–3').. In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds that link nucleotides together to form nucleic acids.
RNase R, or Ribonuclease R, is a 3'-->5' exoribonuclease, which belongs to the RNase II superfamily, a group of enzymes that hydrolyze RNA in the 3' - 5' direction. RNase R has been shown to be involved in selective mRNA degradation, particularly of non stop mRNAs in bacteria. [1] [2] RNase R has homologues in many other organisms.
Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal nuclease, spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an endo-exonuclease that preferentially digests single-stranded nucleic acids.