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In ICP8, the herpes simplex virus (HSV-1) single-strand DNA-binding protein (ssDNA-binding protein (SSB)), the head consists of the eight alpha helices.The front side of the neck region consists of a five-stranded beta-sheet and two alpha helices, whereas the back side is a three-stranded beta-sheet The shoulder part of the N-terminal domain contains an alpha-helical and beta-sheet region. [1]
Single-stranded DNA is produced during all aspects of DNA metabolism: replication, recombination, and repair. As well as stabilizing this single-stranded DNA, SSB proteins bind to and modulate the function of numerous proteins involved in all of these processes. Active E. coli SSB is composed of four identical 19 kDa subunits. Binding of single ...
20823 Ensembl ENSG00000138385 ENSMUSG00000068882 UniProt P05455 P32067 RefSeq (mRNA) NM_003142 NM_001294145 NM_001110145 NM_009278 NM_001355265 RefSeq (protein) NP_001281074 NP_003133 NP_001103615 NP_033304 NP_001342194 Location (UCSC) Chr 2: 169.79 – 169.81 Mb Chr 2: 69.69 – 69.7 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Sjögren syndrome type B antigen (SS-B) also known ...
The SSB protein interacts with DNA helicases PriA and RecG to recover stalled DNA replication forks. These enzymes must bind to the SSB-helicase to be loaded onto stalled forks. Thermal sliding and DNA duplex binding are possibly supported by the wedge domain of RecG's association with the SSB linker.
Replication protein A (RPA) is the major protein that binds to single-stranded DNA (ssDNA) in eukaryotic cells. [ 1 ] [ 2 ] In vitro , RPA shows a much higher affinity for ssDNA than RNA or double-stranded DNA. [ 3 ]
Providing four calories per gram, protein's recommended dietary allowance (RDA) for a sedentary adult is 0.8 grams per kilogram or 0.36 grams of protein per pound of body weight.
The RPA process employs three core enzymes – a recombinase, a single-stranded DNA-binding protein (SSB) and strand-displacing polymerase. Recombinases are capable of pairing oligonucleotide primers with homologous sequence in duplex DNA. [1] SSB bind to displaced strands of DNA and prevent the primers from being displaced.
To qualify, they have to have limited amounts of saturated fat, sodium and added sugar and contain foods from the major dietary groups such as fruits, vegetables, protein and dairy.