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The SI unit of molar absorption coefficient is the square metre per mole (m 2 /mol), but in practice, quantities are usually expressed in terms of M −1 ⋅cm −1 or L⋅mol −1 ⋅cm −1 (the latter two units are both equal to 0.1 m 2 /mol).
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By measuring near-normal incident reflectance (R) of the film (from 190 to 1000 nm), and analyzing R using the Forouhi–Bloomer dispersion equations, the film can be completely characterized. The Forouhi–Bloomer dispersion equations in combination with Rigorous Coupled-Wave Analysis (RCWA) have also been used to obtain detailed profile ...
The molar extinction coefficient of Hb has its highest absorption peak at 420 nm and a second peak at 580 nm. Its spectrum then gradually decreases as light wavelength increases. On the other hand, H b O 2 {\displaystyle HbO2} shows its highest absorption peak at 410 nm, and two secondary peaks at 550 nm and 600 nm.
Thus, for a fixed path length, UV-Vis spectroscopy can be used to determine the concentration of the absorber in a solution. It is necessary to know how quickly the absorbance changes with concentration. This can be taken from references (tables of molar extinction coefficients), or more accurately, determined from a calibration curve.
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Extinction coefficient refers to several different measures of the absorption of light in a medium: Attenuation coefficient , sometimes called "extinction coefficient" in meteorology or climatology Mass extinction coefficient , how strongly a substance absorbs light at a given wavelength, per mass density
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