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Since the reaction requires a free carbonyl group, only "reducing sugars" participate. Sucrose, which is nonreducing, does not form an osazone. A typical reaction showing the formation of an osazone. D-glucose reacts with phenylhydrazine to give glucosazone. The same product is obtained from fructose and mannose. General steps in osazone formation
Structure of the hydrazone functional group. Hydrazones are a class of organic compounds with the structure R 1 R 2 C=N−NH 2. [1] They are related to ketones and aldehydes by the replacement of the oxygen =O with the = N−NH 2 functional group.
The reagent used can be any substance that gives S 2− ions in such solutions; most commonly used are hydrogen sulfide (at 0.2-0.3 M), thioacetamide (at 0.3-0.6 M), addition of hydrogen sulfide can often prove to be a lumbersome process and therefore sodium sulfide can also serve the purpose. The test with the sulfide ion must be conducted in ...
Molisch test (using α-napthol) indicating a positive result (see purple ring). Molisch's test is a sensitive chemical test, named after Austrian botanist Hans Molisch, for the presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid or hydrochloric acid to produce an aldehyde, which condenses with two molecules of a phenol (usually α-naphthol, though other ...
The reagents consist of resorcinol and concentrated hydrochloric acid: The acid hydrolysis of polysaccharide and oligosaccharide ketoses yields simpler sugars followed by furfural. [1] The dehydrated ketose then reacts with two equivalents of resorcinol in a series of condensation reactions to produce a molecule with a deep cherry red color.
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Chelate complex of the Chen-Kao reaction starting from (1S,2S)-pseudoephedrine. The Chen-Kao reaction (named after Ko Kuei Chen and Chung-Hsi Kao, Madison, Wisconsin 1926) [1] [2] is a chemical method for determining the presence of pseudoephedrine, ephedrine, and similar phenylalkylamines. [3]
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